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Papers Patents
Papers from the
| 2015
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Biomembrane Lab,
University of Michigan
   
2015
68. "Hydrogel-assisted functional reconstitution of human P-glycoprotein (ABCB1) in giant liposomes."
Horger K.S., Liu H., Rao D.K., Shukla S., Sept D., Ambudkar S.V., Mayer M.*
Biochimica et Biophysica Acta (BBA), 2015, 1848, 643-653. Abstract, Full Text
  This work describes agarose-film-based formation of giant proteoliposomes containing P-glycoprotein (P-gp) from small proteoliposomes. By measuring the amount of the fluorescent substrate rhodamine 123 inside and outside of these proteoliposomes, the authors were able to determine the rate of passive diffusion as well as active transport by reconstituted P-gp. The results presented here show that P-gp is functionally after following reconstitution and the transport rate increases in the presence of ATP and decreases in the presence of the competitive inhibitor verapamil.
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2014
67. "Translocation of DNA Through Nanopores in Carbon Nano-, Silicon-Nitride- and Lipid-Coated Membranes."
Sischka A., Galla L., Meyer A.J., Spiering A., Mayer M., Hall A.R., Beyer A., Reimann P., Gölzhäuser A., Anselmetti D.
2014, submitted
66. "Real-time shape determination and 5-D fingerprinting of single proteins."
Yusko E.C., Bruhn B.R. (equal contribution), Eggenberger O., Rollings R.C., Walsh N.C., Nandivada S., Pindrus M., Sept D., Li J., Kalonia D.S., Mayer M.*
2014, submitted
65. "Laser nanosurgery reveals the role of local niche signals in asymmetric stem cell divisions in the Drosophila testis."
Chen C., Hunt A.J., Mayer M., Yamashita Y.M.*
2014, submitted
64. "A combination of curcumin with either gramicidin or ouabain selectively kills cells that express the multidrug resistance-linked ABCG2 transporter."
Rao D.K., Liu H., Ambudkar S.V., Mayer M.*
J Biol Chem, 2014, 289, 31397-31410. Abstract, Full Text
  This paper reports a collateral sensitivity strategy to kill selectively multidrug resistant cells that overexpress the ABCG2 transporter via a mechanism of ATP-depletion. This strategy may serve as a potential treatment or inspire similar therapies against drug-resistant cancer cells.
63. "Hydrodynamic slip on DNA observed by optical tweezers-controlled translocation experiments with solid-state and lipid-coated nanopores."
Galla L., Meyer A.J., Spiering A., Sischka A., Mayer M., Hall A.R., Reimann P., Anselmetti D.*
Nano Lett, 2014, 14, 4176-4182. Abstract, Full Text
  This paper uses optical tweezers to characterize the threading force on a single dsDNA molecule inside silicon nitride nanopores. Reducing the nanopore diameter or coating the nanopore with a lipid coating is shown to increase threading force by decreasing electroosmotic flow. The authors present a quantitative model that incorporates hydrodynamic slip to explain their results.
62. "Dual-pore glass chips for cell-attached single-channel recordings."
Bruhn B.R., Liu H., Schuhladen S., Hunt A.J., Mordovanakis A., Mayer M.*
Lab Chip, 2014, 14, 2410-2417. Abstract, Full Text
  Generally, planar patch-clamp platforms are not well-suited for single-channel recordings due to excess noise resulting from low seal resistances and the use of substrates with poor dielectric properties. Here, femtosecond laser ablation was used to fabricate dual-pore glass chips optimized for use in such recordings. Patch-clamp experiments with these chips consistently achieved high seal resistances, maintained gigaseals for up to 6 hours, achieved the lowest RMS noise ever reported for a planar patch-clamp platform, and enabled single-channel recordings in the cell-attached configuration that are comparable to those obtained by conventional patch-clamp.
  This article was featured on the inside cover of Lab on a Chip (Issue 14) and was selected as a LOC HOT article.  
61. "Osmosis-Based Pressure Generation: Dynamics and Application."
Bruhn B.R., Schroeder T.B.H. (equal contribution), Li S., Billeh Y.N., Wang K.W., Mayer M.*
PLOS ONE, 2014, 9, e91350 (10 pgs). Abstract, Full Text
  This work describes the dynamics of osmotically-driven pressure generation in a membrane bound-compartment while taking into account relevant parameters such as solute dilution and membrane fouling. The authors developed an open source tool for numerically simulating pressure versus time curves; simulations match well with experimental data, reveal unintuitive results regarding the dynamics of pressure generation, and provide insight into pressure generation in plant cells. Finally, the authors demonstrate two potential uses of osmosis-based pressure generation: actuation of a soft robot and continuous volume delivery over prolonged durations.
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2013
60. "Engineered ion channels as emerging tools for chemical biology."
Mayer M.* and Yang J.*
Acc. Chem. Res., 2013, 46, 2998-3008. Abstract, Full Text
  This paper describes uses of ion channels and pore-forming peptides such as gramicidin in chemical biology. The authors discuss methods for preparing synthetically tailored ion channels and compare membrane environments or systems that make it possible to quantify the response of ion channels to biochemical processes of interest.
59. "Centrosome-dependent asymmetric inheritance of the midbody ring in Drosophila germline stem cell division."
Salzmann V., Chen C., Chiang C.-Y.A., Tiyaboonchai A., Mayer M., Yamashita Y.M.*
Mol. Biol. Cell., 2013, 25, 267-275. Abstract, Full Text
  In cytokinesis, only one daughter cell inherits the midbody ring. This paper investigates the role of this asymmetry in the context of dividing Drosophila germline stem cells, wherein one stem cell and one differentiating daughter are produced. The authors suggest that the midbody ring does not inherently dictate stem cell identity but may serve as a platform for asymmetric segregation of certain factors.
58. "A Model for the Interfacial Kinetics of Phospholipase D Activity on Long-Chain Lipids"
Majd S., Yusko E.C., Yang J., Sept D., Mayer M.*
Biophysical Journal, 2013, 105, 146-153. Abstract, Full Text
  This paper examines the kinetics of the membrane-active enzyme phospholipase D (PLD). PLD is important in cell signaling and catalyzes the hydrolysis of the phosphodiester bond in phospholipids. Here, the authors characterize the kinetics of PLD with planar lipid bilayers under well-defined experimental conditions. Furthermore, the authors present an extension of a basic kinetic model for interfacial catalysis.
  This article was featured on the cover of Biophysical Journal (Vol. 105, #1) and highlighted in its "New and Notable" article.  
57. "A Tetra(Ethylene Glycol) Derivative of Benzothiazole Aniline Enhances Ras-Mediated Spinogenesis."
Megill A., Lee T., DiBattista A.M., Song J.M., Spitzer M.H., Rubinshtein M., Habib L.K., Capule C.C., Mayer M., Turner R.S., Kirkwood A., Yang J., Pak D.T.S., Lee H.K.*, Hoe H.S.*
J. Neurosci., 2013, 33, 9306 (13 pgs). Abstract, Full Text
  The tetra(ethylene glycol) derivative of benzothiazole aniline (BTA-EG4) is a potential therapeutic agent for preventing amyloid-β-induced cell toxicity in Alzheimer's disease. Here, the biological effects of BTA-EG4 on synaptic function are investigated. The authors show that BTA-EG4 acts to increase Ras-mediated spinogenesis, bolstering its promise as a potential therapeutic agent.
56. "Effects of interleaflet coupling on the morphologies of multicomponent lipid bilayer membranes"
Funkhouser C.M., Mayer M., Solis F.J., Thornton K.*
J. Chem. Phys., 2013, 138, 024909 (12 pgs). Abstract, Full Text
  This paper examines the effect of interleaflet interactions on the dynamics and stationary states of phase-separating multicomponent lipid bilayer membranes using a computational model that employs a phase-field method.
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2012
55. "Synthetic lipid membrane channels formed by designed DNA nanostructures."
Langecker M., Arnaut V., Martin T.G., List J., Renner S., Mayer M., Dietz H.*, Simmel F.C.*
Science, 2012, 338, 932-936. Abstract, Full Text
  Inspired by biological pores such as α-hemolysin, this paper presents an engineered, synthetic ion-channel. The synthetic ion channel was realized through the self-assembly capabilities of nucleic acids using DNA-oragami strategies, and it demonstrates a large leap toward engeineered channels with highly tailorable and specific properties.
  This article was highlighted in a Perspective by Michael Strano in Science and in a RSC News Release.  
54. "Multivariate analyses of amyloid-beta oligomer populations indicate a connection between pore formation and cytotoxicity."
Prangkio P., Yusko E.C., Sept D., Yang J., Mayer M.*
PLOS ONE, 2012, 7, e47261 (10 pgs). Abstract, Full Text
  This paper presents a careful study of aggregates of the Alzheimer's disease related peptide, amyloid-β, and shows that certain aggregates are highly correlated with cell toxicity and ion channel activity in lipid membranes. The results suggest that the ion channel activity of certain aggregates sizes is a mechanism for neuronal cell death in Alzheimer's disease.
  This article was highlighted by UM News (16Oct2012), a radio interview with Prof. Michael Mayer on WILS 1320, and Futurity. Machines Like Us also picked up the UM News piece. More recently, MconneX featured a video of Prof. Mayer discussing this research.  
53. "Spatiotemporally controlled single cell sonoporation."
Fan Z., Liu H., Mayer M., Deng C.X.*
Proc. Natl. Acad. Sci. U. S. A., 2012, 109, 16486-16491. Abstract, Full Text
  This paper quantifies the dynamic process of ultrasound-mediated cell membrane disruption, or sonoporation, and its ability to deliver molecules to the cell interior of single cells using whole-cell patch clamp recordings and fluorescence microscopy. This investigation will help sonoporation become an enabling technology for controlled, intracellular delivery of membrane-impermeant molecules.
52. "Single particle characterization of Aβ oligomers in solution."
Yusko E.C., Prangkio P., Sept D., Rollings R.C., Li J., Mayer M.*
ACS Nano, 2012, 6, 5909-5919. Abstract, Full Text
  This paper demonstrates the potential of resistive-pulse sensing through lipid bilayer-coated nanopores for determining the size of individual amyloid-β oligomers in solution and without chemical modification. By measuring ΔI values due to individual amyloid-β oligomers, protofibrils, or fibers, this method made it possible to account for the large heterogeneity of amyloid-β aggregate sizes.
   
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2011
51. "Peripherally induced human regulatory T cells uncouple Kv1.3 activation from TCR-associated signaling"
Reneer M.C., Estes D.J., Velez-Ortega A.C., Norris A., Mayer M., Marti F.*
Eur. J. Immunol, 2011, 41, 3170-3175. Abstract, Full Text
  This paper describes the uncoupling of the voltage-gated ion channel Kv1.3 from the TCR pathway in a subset of regulatory T cells known as iTregs. iTregs are increasingly recognized as relevant T cell subset, and before they can become a therapeutic target, their molecular basis for development needs to be understood.
50. "Self-assembled, cation-selective ion channels from an oligo(ethylene glycol) derivative of benzothiazole aniline"
Prangkio P., Rao D.K., Lance K.D., Rubinshtein M., Yang J.,Mayer M.*
Biochimica et Biophysica Acta (BBA), 2011, 1808, 2877-2885. Abstract , Full Text
  This paper describes the spontaneous formation of well-defined pores in planar lipid bilayers from the selfassembly of a small synthetic molecule that contains a benzothiazole aniline (BTA) group attached to atetra-ethylene glycol (EG4) moiety. Similar to natural anti microbials, this synthetic compound might be appealing as a starting material for development of antibiotics or membrane-permeating moieties for drug delivery.
49. "Using charge to control the functional properties of self-assembled nanopores in membranes"
Macrae M.X., Schlamadinger D., Mayer M.*, Yang J.*
Small, 2011, 7, 2016-2020. Abstract , Full Text
  This paper describes light sensitive, semisynthetic ion channels, which have properties that can be easily and precisely manipulated. Covalent attachment of a photoswitchable spiropyran group to the entrance of a gramicidin A ion channel enables reversible control of both conductance and channel lifetime, which show opposite trends in behavior at high and low salt concentrations.
48. "Nanopore recordings to quantify activity-related properties of proteins"
Yusko E.C., Billeh Y.N., Yang J., Mayer M.* p. 203-225.
Nanopores: Sensing Fundamental Biological Interactions at the Single Molecule Level.
Eds. Samir I. and Bashir R. 1st Edition. 370 pp. (Springer Publishing Co., New York, 2011). About this Book, Chapter PDF
47. "Controlling protein translocation through nanopores with bio-inspired fluid walls"
Yusko E.C., Johnson J.M., Majd S., Prangkio P., Rollings R.C., Li J., Yang J.*,
Mayer M.*
Nature Nanotech., 2011, 6, 253-260. Abstract , Full Text
  Many challenges have limited the utility of nanopores as a sensor element; challenges include preventing the pores from clogging, imparting specificity to the pores for specific analytes, and enabling controlled and predictive translocation of analytes.  Mimicking the lipid-coated nanopores of insects, this work demonstrates, for the first time, the capture, concentration, and translocation of analytes in a quantitative and predictive fashion.
  This article was highlighted in Nature (3Mar2011), Nature Nanotechnology (6Apr2011), Nature Biotechnology (7Jun2011), Chemistry World (20Feb2011), UM News Service (28Feb2011), Cosmos (1Mar2011), and Futurity (1Mar2011).  
   
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2010
46. "Current oscillations generated by precipitate formation in the mixing zone between two solutions inside a nanopore."
Yusko E.C., Billeh Y.N., Mayer M.*
Journal of Physics: Condensed Matter, 2010, 22, 454127 (9 pgs). Abstract , Full Text
  This paper describes a unique method for generating oscillations in the ionic current through synthetic nanopores. The oscillations are due to the precipitation of small organic molecules inside the pore followed by the suprising removal of the precipitate from the pore. Mechanisms for the ionic current oscillations as well as potential applications are dicsussed.
45. "Applications of biological pores in nanomedicine, sensing and nanoelectronics"
Majd S., Yusko E.C., Billeh Y.N., Macrae M.X., Yang J., Mayer M.*
Current Opinion in Biotechnology, 2010, 21, 439-476. Abstract , Full Text
  This review appears in a themed issue regarding Nanobiotechnology. It reviews the current state of applications of pore-forming peptides and proteins in nanomedicine, sensing, and nanoelectronics. In the future these pores may be applied for single strand DNA sequencing and for generating bio-inspired, and possibly, biocompatible visual detection systems and batteries.
44. "Nanoscale ionic diodes with tunable and switchable rectifying behavior"
Macrae M.X., Blake S., Mayer M.*, Yang J.*
J. Am. Chem. Soc., 2010, 132, 1766-1777. Abstract , Full Text
  This paper reports a modular strategy for constructing self-assembled ionic diodes in membranes using semisynthetic ion channel-forming peptides with internal diameters of less than 1 nm.
43. "Electroosmotic flow can generate ion current rectification in nano- and micropores"
Yusko E.C., An R., Mayer M.*
ACS Nano, 2010, 4, 477-487. Abstract , Full Text
  This paper shows that under the right conditions electroosmotic flow is significant in nano- and micropores. The electroosmotic flow of solutions with a different conductance can then result in ion current rectification. Thus this extends the concept of ion current rectification in fluid-filled pores to the microscale.
 
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2009
42. "Reactive derivatives of gramicidin enable light- and ion-modulated ion channels"
Macrae M.X., Blake S., Mayer T., Mayer M.*, Yang J.*
Proc. SPIE, 2009, 7397, 11. Full Text
  This paper discusses the development of gA-based sensors for detecting external factors such as metal ions in solution or for detecting specific wavelengths of light. We propose that gA-based ion channel sensors offer tremendous potential for ultra sensitive functional detection since a single chemical modification of each individual sensing element can lead to readily detectable changes in channel conductance.
41. "Gramicidin pores report the activity of membrane-active enzymes"
Majd S., Yusko E.C., MacBriar A.D., Yang J.* Mayer M.*
J. Am. Chem. Soc., 2009, 131, 16119-16126. Abstract , Full Text
  Phospholipases constitute a ubiquitous class of membrane-active enzymes that play a key role in cellular processes, and aberrant phospholipase activity is implicated in a range of diseases. Assays designed to improve the understanding of phospholipase catalysis and to accelerate pharmaceutical and biotechnological applications are in demand. This paper describes a novel approach to monitor, in situ and in real-time, the activity of phospholipase D and phospholipase C on planar lipid bilayers.
40. "A semi-synthetic ion channel platform for detection of phosphatase and protease activity"
Macrae M.X, Blake S., Jiang X., Capone R., Estes D.J., Mayer M.*,
Yang J.*
ACS Nano, 2009,11, 3567-3580. Abstract , Full Text
  This paper describes the detection and characterization of two enzymes, alkaline phosphatase and anthrax lethal factor. This method uses single ion channel conductance measurements to follow the enzyme-catalyzed hydrolysis of a substrate attached to gramicidin A in the presence of these enzymes. Applying this concept, the authors determined Km and Kcat for alkaline phosphatase and illustrated the utility of ion channel-based sensing for detection of a potential biowarfare agent.
39. "Amyloid-beta-induced ion flux in artificial lipid bilayers and neuronal cells: resolving a controversy"
Capone R., Quiroz F.G., Prangkio P., Saluja I., Sauer A.M., Bautista
M.R., Turner R.S., Yang J., Mayer M.*,
Neurotoxicity Research, 2009, 16, 1-13. Abstract , Full Text
  Understanding the pathogenicity of amyloid-beta (AB) peptides constitutes a major goal in research on Alzheimers disease (AD). One hypothesis entails that AB peptides induce uncontrolled, neurotoxic ion flux through cellular membranes. The exact biophysical mechanism of this ion flux is, however, a subject of an ongoing controversy which has attenuated progress toward understanding the importance of AB-induced ion flux in AD. The work presented here addresses two prevalent controversies regarding the nature of transmembrane ion flux induced by AB peptides.
  This article was highlighted in the University of Michigan News, in Science Daily, and in e! Science News (April 15, 2009).  
38. "Films of agarose enable rapid formation of giant liposomes in solutions of physiological ionic strength"
Horger K.S., Estes D.J., Capone R., Mayer M.*
J. Am. Chem. Soc., 2009, 131, 1810-1819. Abstract , Full Text
  This paper describes a method to form giant liposomes in solutions of physiologic ionic strength, such as phosphate buffered saline (PBS) or 150 mM KCl. Formation of these cell-sized liposomes proceeded from hybrid films of partially dried agarose and lipids. Hydrating the films of agarose and lipids in aqueous salt solutions resulted in swelling and partial dissolution of the hybrid films and in concomitant rapid formation of giant liposomes in high yield.
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2008
37. "Generating arrays with high content and minimal consumption of functional membrane proteins"
Majd S., Mayer M.*
J. Am. Chem. Soc., 2008, 130, 16060-16064. Abstract , Full Text
  This paper introduces a widely accessible and straightforward technique for fabricating membrane protein arrays. It can fabricate more than 30 identical copies of a membrane protein array while requiring only femtomoles of protein.
36. "Chemically reactive derivatives of gramicidin A for developing ion channel-based nanoprobes"
Blake S., Capone R., Mayer M.*, Yang J.*
Bioconjugate Chem., 2008, 19, 1614-1624. Abstract , Full Text
  This paper describes a practical, synthetic route to generate C-terminally modified derivatives of gramicidin A (gA), an ion channelforming peptide, through the use of two chemically reactive gA-based building blocks. We demonstrate that derivatives of gA can be used for studying the change in conductance through gA upon performing a "click" reaction on an azide moiety attached to the gA pore. We also demonstrate that these gA-based building blocks can be used as sensors for protein-ligand interactions.
35. "Detection and quantification of lipid membrane binding on silca micro-tube resonator sensor"
Ling T., Majd S., Mayer M., Guo L. J.*
Proc. SPIE, 2008, 6862, 68620B1-68620B8. Abstract , Full Text
  This paper studies the binding of a lipid membrane onto a micro-tube sensor that is probed by a prism coupling technique. The presented method affords a sensitive technique to detect binding of proteins to a lipid bilayer.
34. "Noise and bandwidth of current recordings from submicrometer pores and nanopores"
Uram J. D., Ke K., Mayer M.*
ACS Nano, 2008, 2, 857-872. Abstract , Full Text
  In this report, we present a detailed theoretical and experimental study on the noise and signal bandwidth of current recordings from glass and polyethylene terephthalate (PET) membranes that contain a single submicrometer pore or nanopore. This work will be useful for minimizing noise and achieving accurate recordings in nanopore-based experiments.
33. "High-throughput profiling of ion channel activity in primary human lymphocytes"
Estes D.J., Memarsadeghi S. (D.J.E. and S.M. contributed equally), Lundy S.K., Mikol D.D., Fox D.A., Mayer M.*
Anal. Chem., 2008, 80, 3728-3735. Abstract , Full Text
  This paper presents a high-throughput method to quantify the functional activity of potassium (K+) ion channels in primary human lymphocytes. This work affords insight into using Kv1.3 activity as a marker of T cell activation, and also provides a general methodology for high-throughput profiling of voltage-gated ion channel activity in a range of primary cell types.
  This article was highlighted as a Research Profile in the June 1, 2008 issue of Analytical Chemistry  
32. "Ultrafast laser fabrication of submicrometer pores in borosilicate glass"
An R., Uram J.D., Yusko E.C., Ke K., Mayer M., Hunt A.J.*
Optics Letters, 2008, 33, 1153-1155. Abstract , Full Text
  This paper demonstrate rapid fabrication of submicrometer-diameter pores in borosilicate glass using femtosecond laser machining and subsequent wet-etch techniques. The technique presented in this work affords repeatable and direct fabrication of high-quality pores with diameters between 400-800 um, with smaller pores possible with a subsequent deposition of SiO2 by PECVD.
31. "Characterization of changes in the viscosity of lipid membranes with the molecular rotor FCVJ"
Nipper M.E., Majd S., Mayer M., Lee J., Theodorakis E. A., Haidekker M. A.*
Biochim. Biophys. Acta, 2008, 1778, 1148-1153. Abstract , Full Text
  The purpose of this study is to examine the fluorescent behavior of FCVJ in model membranes exposed to various agents of known influence on membrane viscosity, such as alcohols, dimethyl sulfoxide (DMSO), cyclohexane, cholesterol, and nimesulide.
"Using ion channel-forming peptides to quantify protein-ligand interactions"
Mayer M.*, Semetey V., Gitlin I., Yang J., Whitesides G.M.*
J. Am. Chem. Soc., 2008, 130, 1453-1465. Abstract, Full Text
  This paper proposes a method for sensing affinity interactions by triggering disruption of selfassembly of ion channel-forming peptides in planar lipid bilayers. It shows that the binding of a derivative of alamethicin carrying a covalently attached sulfonamide ligand to carbonic anhydrase II (CA II) resulted in the inhibition of ion channel conductance through the bilayer. This method gave a dissociation constant of 0.2 µM for the binding of CA II to alamethicin-sulfonamide in the bilayer recording chamber.
   
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2007    
30. "Designing nanosensors based on charged derivatives of gramicidin A"
Capone R., Blake S., Rincon Restrepo M., Yang J.*, Mayer M.*
J. Am. Chem. Soc., 2007, 129, 9737-9745. Abstract , Full Text
  This paper discusses five key design parameters to optimize the performance of chemomodulated ion channel sensors based on derivatives of gramicidin A. Charge-based ion channel sensors offer tremendous potential for ultrasensitive functional detection since a single chemical modification of each individual sensing element can lead to readily detectable changes in channel conductance.
29. "Estimation of solid phase affinity constants using resistive-pulses from functionalized nanoparticles"
Uram J. D., Mayer M.*
Biosensors and Bioelectronics, 2007, 22, 1556-1560. Abstract , Full Text
  This paper describes a method for estimating the solid phase affinity constant of antibodies by using resistive-pulse (Coulter counting) data from spherical nanoparticles that expose antigens.
   
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2006    
28. "Monitoring chemical reactions by using ion channel-forming peptides"
Blake S., Mayer T., Mayer M.*, Yang J.*
ChemBioChem, 2006, 7 , 433-435. Abstract , Full Text
  This paper presents a method for monitoring chemical reactions on individual molecules by measuring functional changes in ion channel peptides modified with different chemical groups.
27. "Submicrometer pore-based characterization and quantification of antibody-virus interactions"
Uram J. D., Ke K., Hunt A. J., Mayer M.*
Small, 2006, 2, 967-972. Abstract , Full Text
  This paper describes the use of a submicrometer pore-based resistive-pulse sensor to i) detect a specific virus or a virus-specific antibody in solution, ii) probe the ability of an antibody to immunoprecipitate the virus, iii) determine the number of antibodies bound to individual virus particles, and iv) monitor the assembly of nanoparticles on templates (here antibodies on viruses) in situ.
26. "Assays for studying annexin binding to artificial lipid bilayers"
Majd S., Estes D. J. (S.M. and D.J.E. contribued equally), Mayer M.*
Calcium Binding Proteins, 2006, 1:1, 26-29. Abstract , Full Text
  This paper describes two new techniques for investigating the interactions of annexins with lipid bilayers of well-defined compositions, one approach using hydrogel-stamped supported lipid bilayers, and the other approach using surface-attached giant liposomes in microfluidic chambers.
25. "Triggering and visualizing the aggregation and fusion of lipid membranes in microfluidic chambers"
Estes D. J., Lopez S. R., Fuller A. O., Mayer M.*
Biophysical Journal, 2006, 91, 233-243. Abstract , Full Text
  We present a method that makes it possible to trigger, observe, and quantify membrane aggregation and fusion of giant liposomes in microfluidic chambers.
24. "Label-free affinity assays by rapid detection of immune complexes in submicrometer pores"
Uram J. D., Ke K., Hunt A. J., Mayer M.*
Angew. Chem. Int. Ed., 2006, 45, 2281-2285. Abstract , Full Text
  We present a method that uses a submicrometer pore to detect and characterize immune complexes consisting of proteins such as staphylococcal enterotoxin B (an agent with bioterrorism potential) and polyclonal antibodies.
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2005
23. "Hydrogel-stamping of arrays of supported lipid bilayers with various lipid compositions for screening of drug-membrane and protein-membrane interactions"
Majd S., Mayer M.*
Angew. Chem. Int. Ed., 2005, 44, 6697-6700. Abstract , Full Text
  Herein we describe a rapid, reproducible, and straightforward method to form copies of functional membrane arrays with various lipid compositions and the application of these arrays for the screening of drug–membrane and protein–membrane interactions.
  This article was highlighted in Nature Chemical Biology (November 6, 2005) and UM News Service (November 21, 2005).
22. "Giant liposomes in physiological buffer using electroformation in a flow chamber"
Estes D. J., Mayer M.*
Biochim. Biophys. Acta, 2005, 1712, 152-160. Abstract , Full Text
  We describe a method to obtain giant liposomes (diameter 10–100 um) in solutions of high ionic strength to perform a membrane-binding assay under physiological conditions.
21. "Electroformation of giant liposomes from spin-coated films of lipids"
Estes D. J., Mayer M.*
Colloids and Surf. B., 2005, 42, 115-123. Abstract , Full Text
  This paper describes spin-coating of solutions of lipids and using the resulting thin films for electroformation of giant liposomes.
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  1. Michael Mayer's Papers from the Langer Lab, MIT (in collaboration with the Whitesides Lab)
  2. 20. "Direct Patterning of mammalian cells onto porous tissue engineering substrates using agarose stamps"
    Stevens M. M., Mayer M. (S.M.M. and M.M. contribued equally), Anderson D. G., Weibel D. B., Whitesides G. M., Langer R.*
    Biomaterials, 2005, 26, 7636-7641. Abstract , Full Text (pdf)
  3. Michael Mayer's Papers from the Whitesides Lab, Harvard University
  4. 19. "Using ratchets and sorters to fractionate motile cells of Escherichia coli by length"
    Hulme S. E., DiLuzio W. R., Shevkoplyas S. S., Turner L., Mayer M., Berg H. C., Whitesides G. M.
    Lab chip, 2008, 8, 1888-1895. Abstract, Full Text (pdf)
  5. 18. "Using ion channel-forming peptides to quantify protein-ligand interactions"
    Mayer M.*, Semetey V., Gitlin I., Yang J., Whitesides G.M.*
    J. Am. Chem. Soc., 2008, 130, 1453-1465. Abstract, Full Text (pdf)
  6. 17. "Microoxen: microorganisms to move microscale loads"
    Weibel D. B., Garstecki P., Ryan D., DiLuzio W., Mayer M., Seto J. E., Whitesides G. M.*
    Proc. Nat. Acad. Sci. USA, 2005, 102, 11963-11967. Abstract, Full Text (pdf)
  7. 16. "Escherichia coli drive on the right"
    DiLuzio W. R., Turner L., Mayer M., Garstecki P., Weibel D. B., Berg H. C., Whitesides G. M.*
    Nature, 2005, 435, 1271-1274. Abstract, Full Text (pdf), Supplementary Movie (mpeg)
  8. 15. "A bacterial printing press that regenerates its ink: contact printing bacteria using hydrogel stamps"
    Weibel D. B., Lee A., Mayer M., Brady S. F., Bruzewicz D., Yank J., DiLuzio W., Clardy J., Whitesides G. M.*
    Langmuir, 2005, 21(14), 6436-6442. Abstract , Full Text (pdf), Cover Image
  9. 14. "Micropatterned agarose gels for stamping arrays of proteins and gradients of proteins"
    Mayer M., Yang J., Gracias D., Whitesides G. M.*
    Proteomics, 2004, 4, 2366-2376. Abstract , Full Text (pdf)
  10. 13. "Self-Assembled Aggregates of IgGs as Templates for the Growth of Clusters of Gold Nanoparticles"
    Yang J., Mayer M., Kriebel J. K., Garstecki P., Whitesides. G. M.*
    Angew. Chem. Int. Ed., 2004, 43, 1555-1558. Abstract , Full Text (pdf)
  11. 12. "Microfabricated Teflon membranes for low-noise recordings of ion channels in planar lipid bilayers"
    Mayer M.*, Kriebel J. K., Tosteson M. T., Whitesides G. M.*
    Biophys. J., 2003, 85, 2684-2695. Abstract , Full Text (pdf)
  12. 11. "Significance of charge regulation in the analysis of protein charge ladders"
    Gitlin I., Mayer M., Whitesides G. M.*
    J. Phys. Chem. B., 2003, 107, 1466-1472. Abstract , Full Text (pdf)
  13. 10. "Membraneless vanadium redox fuel cell using laminar flow"
    Ferrigno R., Stroock A. D., Clark T. D., Mayer M., Whitesides G. M.*
    J. Am. Chem. Soc., 2002, 124, 12930-12931. Abstract , Full Text (pdf)
  14. Michael Mayer's Papers from Swiss Federal Institute of Technology, Lausanne, Switzerland
  15. 9. "Highly electrically insulating tethered lipid bilayers for probing the function of ion channel proteins"
    Terrettaz S., Mayer M., Vogel H.
    Langmuir, 2003, 19, 5567-5569. Abstract , Full Text (pdf)
  16. 8. "Reversible immobilization of peptides: surface modification and in situ detection by ATR-FTIR spectroscopy"
    Rigler P. H. A., Ulrich W.-P., Hoffmann P, Mayer M., Vogel H.*
    ChemPhysChem, 2003, 4, 268-275. Abstract , Full Text (pdf)
  17. 7. "Monitoring the formation of densely packed recombinant receptors during clustering in the plasma membranes of live biological cells"
    Pick H. M., Preuss A. K., Mayer M., Wohland T., Hovius R., Vogel H.*
    Biochemistry, 2003, 42, 877-884. Abstract , Full Text (pdf)
  18. 6. "Functional analysis of ion channels: Planar patch clamp and impedance spectroscopy of tethered lipid membranes"
    Mayer M., Terrettaz S., Giovangrandi L., Stora T., Vogel H.*
    In: Biosensors - A practical Approach, 2nd Ed., Oxford University Press, Oxford, UK, 2003. Link , Full Text (pdf)
  19. 5. "A chip-based biosensor for the functional analysis of single ion channels"
    Schmidt C., Mayer M., Vogel H.*
    Angew. Chem. Int. Ed., 2000, 39, 3137-3140. Abstract , Full Text (pdf)
  20. 4. "Immunoaffinity screening with capillary electrochromatography"
    Mayer M., Muscate-Magnussen A., Vogel H., Ehrat M., Bruin G. J. M.*
    Electrophoresis, 2002, 23, 1255-1262. Abstract , Full Text (pdf)
  21. Michael Mayer's Papers from Novartis Pharma AG, Basel, Switzerland
  22. 3. "Fritless capillary electrochromatography"
    Mayer M., Rapp E., Marck C., Bruin G. J. M.*
    Electrophoresis, 1999, 20, 43-49. Abstract , Full Text (pdf)
  23. Michael Mayer's Papers from the University of Washington, Seattle
  24. 2. "Flow injection based renewable electrochemical sensor system"
    Mayer M., Ruzicka J.*
    Anal. Chem., 1996, 68, 3808-3814. Abstract , Full Text (pdf)
  25. Michael Mayer's Papers from Federal Institute for Biotechnology, Braunschweig, Germany
  26. 1. "Automated determination of lactulose in milk using an enzyme reactor and flow analysis with integrated dialysis"
    Mayer M., Genrich M., Kuennecke W., Bilitewski U.*
    Anal. Chim. Acta, 1996, 324, 37-45. Abstract , Full Text (pdf)
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Property of the Biomembrane lab, University of Michigan, 2005
Developed by: Dan Estes, Ph.D. Updated by: Brandon Bruhn

This material is based upon work supported by the National Science Foundation under Grant No. 0449088.

Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.