The Takayama Lab: Bio/Nano/Molecular Biotechnology Research

Microfluidics: The following are some of the microfluidic research areas undertaken in the Takayama Lab:

Microfluidic Flow Manipulation and Applications

Cell Culture on a Chip

Embryo Culture on a Chip and Sperm Sorting

Pulmonary Microfluidics

Reviews:

Jovic, A.; Howell, B.; Takayama, S. “Timing is Everything: Using Fluidics to Understand the Role of Temporal Dynamics in Cellular Systems” Microfluidics Nanofluidics 2009, in press.

Smith, G. D.; Takayama, S. “Gamete and embryo isolation and culture using microfluidics”Theriogenology 2007, 68, Suppl. 1 S190-S195.

Suh, R. S.; Takayama, S.; Smith, G. D. “Microfluidic Applications for Andrology” J. Androl. 2005, 664-670

Huh, D.; Kamotani, Y.; Grotberg, J. B.; Takayama, S. “Microfluidics for Flow Cytometric Analysis of Cells and Particles” Physiol. Meas. 2005, R73-R98.

Suh, R. S.; Phadke, N.; Ohl, D. A.; Takayama, S.; Smith, G. D. "Rethinking Gamete/Embryo Isolation and Culture with Microfluidics" Hum. Reprod. Update 2003, 9, 451-461.

Shim, J.; Bersano-Begey, T. F.; Zhu, X.; Tkaczyk, A.; Linderman, J.J.; Takayama, S. “Micro- and Nanotechnologies for Studying Cellular Function” Curr. Top. Med. Chem., 2003, 3, 687-703.

Kenis, P. J. A.; Ismagilov, R. F.; Takayama, S.; Whitesides, G. M.; Li, S; White, H. S. "Microfabrication Inside Microchannels using Fluid Flow" Acc. Chem. Res. 2000, 33, 841-847.

Microfluidic Flow Manipulation::

Chen, H.; Gu, W.; Cellar, N.; Takayama, S.; Kennedy, R.; Meiners, J.-C. “Electromechanical properties of pressure-actuated PDMS microfluidic push-down valves” Anal. Chem. 2008, 80, 6110-6113.

Tung, Y. C.; Torisawa, Y.; Futai, N.; Takayama, S. “Small volume low mechanical stress cytometry using computer-controlled Braille display microfluidics”Lab Chip 2007, 7, 1497-1503.

Chueh, B.-H.; Huh, D.; Kyrtsos, C.R.; Houssin, T.; Futai, N.; Takayama, S. “Leakage-free bonding of porous membranes into layered microfluidics array systems” Analytical Chemistry 2007, 79, 3504-3508.

Huh, D.; Bahng, J.H.; Ling, Y.B.; Wei, H.-H.; Kripfgans, O.D.; Fowlkes, J.B.; Grotberg, J.B.; Takayama, S. “Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification” Analytical Chemistry 2007, 79, 1369-1376.

Gu, W.; Chen, H.; Tung, Y.-C.; Meiners, J.-C.; Takayama, S. “Multiplexed hydraulic valve actuation using ionic liquid filled soft channels and Braille displays” Applied Physics Letters 2007, 90, Art. No. 033505.

Calderon, A. J.; Heo, Y. S.; Huh, D.; Futai, N.; Takayama, S.; Fowlkes, J. B.; Bull, J. L. “A Microfluidic Model of Bubble Lodging in Microvessel Bifurcations” Appl. Phys. Lett. 2006, 89, Art. No. 244103.

Futai, N.; Gu, W.; Takayama, S. “Rapid Prototyping of Microstructures with Bell-Shaped Cross-Sections and Its Application to Deformation-Based Microfluidic Valves” Adv. Mater. 2004, 16, 1320-1323.

Gu, W.; Zhu, X.; Futai, N.; Cho, B. S.; Takayama, S. “Computerized Microfluidic Cell Culture Using Elastomeric Channels and Braille Displays” Proc. Natl. Acad. Sci. U.S.A. 2004, 101, 15861-15866.

Zhu, X.; Chu, L. Y.; Chueh, B.-H.; Shen, M.; Hazarika, B.; Phadke, N.; Takayama, S. ”Arrays of Horizontally-Oriented Mini-Reservoirs Generate Steady Microfluidic Flows for Continuous Perfusion Cell Culture and Gradient Generation” Analyst 2004, 1026-1031.

Takayama, S.; Ostuni, E.; LeDuc, P. Naruse, K.; Ingber, D. E.; Whitesides, G. M. “Selective Chemical Treatment of Cellular Microdomains Using Multiple Laminar Streams” Chem. Biol., 2003, 10, 123-130.

Lim, D.; Kamotani, Y.; Cho, B.; Mazumder, J.; Takayama, S. "Fabrication of Microfluidics Mixers and Artificial Vasculatures Using a High-Brightness Diode-Pumped Nd:YAG Laser Direct Write Method" Lab Chip 2003, 3, 318-323.

Huh, D.; Tkaczyk, A.H.; Bahng, J.-H.; Chang, Y.; Wei, H.-H.; Grotberg, J.B.; Kim, C.-J.; Kurabayashi, K.; Takayama, S. "Reversible Switching of High-Speed Air-Liquid Two-Phase Flows Using Electrowetting-Assisted Flow-Pattern Change" J. Am. Chem. Soc. 2003, 125, 14678-14679.

Huh, D; Tung, Y-C; Wei, H.-H.Grotberg, J.B.; Skerlos, S.J.; Kurabayashi, K.; Takayama, S. “Use of Air-Liquid Two-Phase Flow in Hydrophobic Microfluidic Channels for Disposable Flow Cytometers” Biomed. Microdev. 2002,4, 141-149.

Sawano, A.; Takayama, S.; Matsuda, M.; Miyawaki, A. “Lateral Propagation of EGF Signaling after Local Stimulation Is Dependent on Receptor Density” Dev. Cell, 2002, 3, 245-257.

Takayama, S.; Ostuni, E.; LeDuc, P. Naruse, K.; Ingber, D. E.; Whitesides, G. M. "Laminar Flows: Subcellular Positioning of Small Molecules" Nature, 2001, 411, 1016.

Takayama, S.; Ostuni, E.; Qian, X.; McDonald, J. C.; Jiang, X.; LeDuc, P.; Wu, M.-H.; Ingber, D. E.; Whitesides, G. M. "Topographical Micropatterning of Poly(dimethylsiloxane) Using Laminar Flows of Liquids in Capillaries" Adv. Mater. 2001, 13, 570-574.

Pulmonary Microfluidics:

Tavana, H.; Huh, D.; Grotberg, J. B.; Takayama, S. “Pulmonary Airways on a Chip” BIOforum Europe 2008, 7-8, 14-16.

Fujioka, H.; Takayama, S.; Grotberg, J. B. “Unsteady propagation of a liquid plug in a liquid-lined straight tube” Phys. Fluids 2008, 20, 062104.

Halpern, D.; Fujioka, H.; Takayama, S.; Grotberg, J. B. “Liquid and surfactant delivery into pulmonary airways” Respir. Physiol. Neurobiol. 2008, 163, 222-231.

Huh, D; Fujioka, H.; Tung, Y.-C.; Futai, N.; Paine, R.; Grotberg, J. B.; Takayama, S. “Acoustically Detectable Cellular-Level Lung Injury Induced by Fluid Mechanical Stresses in Microfluidic Airway Systems”J. Proc. Natl. Acad. Sci. U.S.A. 2007, 104, 18886-18891.

Embryo Culture on a chip and Sperm Sorting:

Smith, G. D.; Takayama, S. “Gamete and embryo isolation and culture using microfluidics”Theriogenology 2007, 68, Suppl. 1 S190-S195.

Inamdar, M. V.; Kim, T.; Chung, Y.-K.; Was, A.; Xiang, X.; Wang, C.-W.; Takayama, S.; Lastoskie, C. M.; Thomas, F. I. M.; Sastry, A. M. “Assessment of Sperm Chemokinesis with Exposure to Jelly Coats of Sea Urchin Eggs and Resact: A Microfluidic Experiment, and Numerical Study”J. Exp. Biol. 2007, 210, 3805-3820.

Suh, R. S.; Takayama, S.; Smith, G. D. “Microfluidic Applications for Andrology” J. Androl. 2005, 664-670

Cho, B.; Schuster, T.G.; Zhu, X.; Chang, D.; Smith, G.D.; Takayama, S. “A Passively-Driven Integrated Microfluidic System for Separation of Motile Sperm” Anal. Chem. 2003, 75, 1671-1675.

Schuster, T.G.; Cho, B.; Keller, L. M.; Takayama, S.; Smith, G. D. “Isolation of Motile Sperm From Semen Samples Using Microfluidics” Reproduc. Biomed. Online. 2003, 7, 75-81.

Suh, R. S.; Phadke, N.; Ohl, D. A.; Takayama, S.; Smith, G. D. "Rethinking Gamete/Embryo Isolation and Culture with Microfluidics" Hum. Reprod. Update 2003, 9, 451-461.

Cell Culture on a Chip:

Shen, Y.-C.; Li, D.; Al-Shoaibi, A.; Bersano-Begey, T.; Chen, H.; Ali, S.; Flak, B.; Perrin, C.; Winslow, M.; Shah, H.; Ramamurthy, P.; Schmedlen, R. H.; Takayama, S.; Barald, K. F. ”Student Team in a University of Michigan Biomedical Engineering Design Course Constructs a Microfluidic Bioreactor for Studies of Zebrafish Development” Zebrafish 2009, in press.

Mehta, G.; Kiel, M. J.; Lee, J. W.; Kotov, N.; Linderman, J. J.; Takayama, S. “Polyelectrolyte-Clay-Protein Layer Films on Microfluidic PDMS Bioreactor Surfaces for Primary Murine Bone Marrow Cultures”Adv. Funct. Mater. 2007, 17, 2701-2709.

Heo, Y.S.; Cabrera, L.M.; Song, J.W.; Futai, N.; Tung, Y.-C.; Smith, G.D.; Takayama, S. “Characterization and resolution of evaporation-mediated osmolality shifts that constrain microfluidic cell culture in poly(dimethylsiloxane) devices” Analytical Chemistry 2007, 79, 1126-1134.

Futai, N.; Gu, W; Song, J. W.; Takayama, S. “Handheld Recirculation System and Customized Media for Microfluidic Cell Culture” Lab Chip. 2006, 6, 149-154.

Song, J. W.; Gu, W.; Futai, N.; Warner, K. A.; Nor, J. E.; Takayama, S. “Computer-Controlled Microcirculatory System for Endothelial Cell Culture and Shearing” Anal. Chem. 2005, 77, 3993-3999.